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Cryo-EM imaging was performed on a Titan Krios at 300 kV accelerating voltage in the Center of Cryo-Electron Microscopy Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences (Shanghai, China)

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You want to pinch on an area of fat, ensure the needle is inserted all the way, and keep the needle injected for a few seconds before pulling it out, so all the medication has been absorbed properly